Corrigendum to: The acute physiological status of white sharks (Carcharodon carcharias) exhibits minimal variation after capture on SMART drumlines.

[This corrects the article DOI: 10.1093/conphys/coz042.].

The acute physiological status of white sharks (Carcharodon carcharias) exhibits minimal variation after capture on SMART drumlines.

Drumlines incorporating SMART (Shark-Management-Alert-in-Real-Time) technology are a new tool used in several bather protection programmes globally. In New South Wales (NSW), Australia, the white shark (Carcharodon carcharias) is a target species for SMART drumlines because they are often involved in attacks on humans. To understand white shark sensitivity to capture and to establish protocols around acceptable timeframes for responding to alerts, 47 juvenile and subadult white sharks were caught on SMART drumlines at five locations off the east coast of Australia. There was no at-vessel mortality during the sampling period. After capture, blood was sampled from each shark to assess its acute physiological status. Of the 18 metabolites investigated, only lactate and aspartate aminotransferase exhibited significant positive relationships with the capture duration on SMART drumlines. These results indicate that the capture process is relatively benign and that the current response times used here are appropriate to minimize long-term negative impacts on released white sharks. Where white sharks are likely to interact negatively with beachgoers, SMART drumlines can therefore be a useful addition to bather protection programmes that also aim to minimize harm to captured animals. Other shark species captured on SMART drumlines should also be investigated to gain broader understanding of potential physiological consequences of using this new technology.

Sublethal effects of angling and release on golden perch Macquaria ambigua: implications for reproduction and fish health.

The present study tested the hypothesis of no delayed sublethal effects of mild angling and release on the feeding, growth, somatic condition and gonadal development of golden perch Macquaria ambigua during gametogenesis. Subsamples of adult M. ambigua (n = 17-21 of 207), originally captured from the wild and stocked into ten 0·1 ha earthen ponds, were angled and released during early and late gametogenesis. Wild samples that were concurrently collected throughout the experiment underwent rapid and synchronous gonadal development and many spawned. While no spawning occurred in the ponds, most M. ambigua underwent normal gonadal development to maturity, including the angled fish. Angled fish also fed, maintained condition and actually grew faster than non-angled captive controls. Although females that were angled during late gametogenesis more readily ingested and retained baited hooks, neither their subsequent condition nor gonadal development was significantly affected. The predominance of null results was attributed to the combined effects of the flexible reproductive strategy of M. ambigua, the benignness of mouth hooking and immediate release, and possible methodological issues arising from differential hooking success of more aggressive and resilient individuals. The findings support earlier catch-and-release research, but contrast with reports of acute reproductive effects following capture and handling for aquaculture broodstock. This discrepancy highlights the need for research to specifically address welfare questions relevant to recreational fisheries across various species and angling scenarios.

Age and growth of mangrove red snapper Lutjanus argentimaculatus at its cool-water-range limits.

This study investigates the age and growth of Lutjanus argentimaculatus at its southern (cooler) range limits in eastern Australia. Specimens were collected from New South Wales and southern Queensland between November 2011 and December 2013. Fork lengths (LF ) ranged from 190 to 1019 mm, and ages ranged from 2+ to 57+ years. Growth was described by the von Bertalanffy growth function with coefficients L∞ = 874·92 mm, K = 0·087 year(-1) and t0 = -2·76 years. Estimates of the instantaneous natural mortality rate (M) ranged from 0·072 to 0·25. The LF (mm) and mass (W; g) relationship was represented by the equation: W=2·647×10-5LF2·92. The maximum age of 57+ years is the oldest reported for any lutjanid and comparisons with tropical studies suggest that the age-based demography of L. argentimaculatus follows a latitudinal gradient. High maximum ages and low natural mortality rates indicate considerable vulnerability to overexploitation at the species' cool-water-range limits. These results demonstrate the need to identify underlying processes driving latitudinal gradients in fish demography.

Clinical signs of barotrauma in golden perch, Macquaria ambigua (Richardson), and associated effects on post-release mortality and health.

This study assessed the effects of different retrieval depths (2, 10 or 20 m), surface intervals (none or 15 min) and release methods (untreated, vented or recompressed) on the incidence of external and internal clinical signs of barotrauma (ECSB and ICSB) and post-release mortality in golden perch, Macquaria ambigua (Richardson). Fish were assessed for ECSB before and after surface intervals and either monitored for mortality over 3 days in two deep cages or killed for internal examination. When all fish were left untreated, short-term mortality increased with retrieval depth from 0% and 4.2% among 2 and 10-m fish, respectively, to 19.2% among 20-m fish; while surface interval only affected the incidence of two ECSB (excess buoyancy and a prolapsed cloaca). Mortality was also greater among 20-m fish that were subjected to a 15-min surface interval and left untreated (22.2%) or vented (22.2%) than those that were recompressed (5.6%). Of the ECSB, only exophthalmia was associated with increased mortality, with half of the affected fish dying. However, many fish retrieved from 10 and 20 m also sustained numerous ICSB, including compressed gonads or vital organs and ruptured or collapsed, haemorrhaging swimbladders that remained deflated for up to 3 days after release.

Resilience of inshore, juvenile snapper Pagrus auratus to angling and release.

This study assessed the mortality of 157 snapper Pagrus auratus (9-29 cm, total length, L(T) ) after being conventionally angled and then released into cages (along with 48 controls) for 4 days off south-eastern Australia. Fatalities were restricted to 12 angled fish (7·6%) and mostly attributed to the ingestion of hooks and especially their subsequent removal, which caused substantial blood loss and immediate death. Hook ingestion was significantly biased towards smaller fish (<21 cm L(T)) and attributed to a lower chance of anglers initially detecting these individuals on the line (allowing them to consume more of the baits). While mortalities might be reduced in future via (1) choosing terminal rigs that promote mouth hooking and (2) cutting the line on any-hook ingested fish, the results nevertheless validate releasing unwanted angled inshore juvenile P. auratus as a means for managing their exploitation.

Effects of salinity and anatomical hook location on the mortality and physiological response of angled-and-released sand whiting Sillago ciliata.

Three experiments were done with sand whiting Sillago ciliata: the first two assessed the short-term mortality and physiological response of individuals after being mouth hooked and then subjected to rapid changes in salinity, while the third experiment investigated their longer-term fate after ingesting hooks (independent of salinity changes). In experiment one, 48 tanks containing a single S. ciliata were randomly assigned as either one of three treatments or a control. The fish in treatments one and two were exposed to salinity changes during their angling and subsequent release while those in treatment three were only subjected to angling and air exposure. Control fish remained untouched. Fish were then monitored for up to 6 days for mortalities before blood samples were taken to determine concentrations of plasma cortisol and glucose. Blood samples were also taken from five wild-caught fish to provide baseline estimates of the above variables. None of the treatment or control fish died over the 6 days, and there were no significant differences in blood cortisol or glucose between treatment, control and wild fish. In experiment two, 102 S. ciliata and 52 experimental tanks were used. The treatments were repeated as above, however, six individuals from each treatment and control group were removed and sampled for blood (and then glucose and cortisol) at 0, 24, 48 and 72 h post release. Some changes in behaviour due to the salinity changes occurred as well as a significant main effect of time for cortisol, with all fish having significantly elevated acute stress at the first sample time. In experiment three, 52 S. ciliata were placed into individual tanks. Twenty-six of these fish were allowed to ingest baited J-hooks, played for 60 s, removed from their tanks, and then released after their lines were cut (50 mm from their mouths). Control fish were not touched. All fish were then monitored over 21 days. Six of the treatment fish died (between 3 h and 14 days), while the remaining hooked fish resumed feeding within 5 days and 25% ejected their hooks (between 1 and 19 days). It was concluded that (1) salinity and mouth hooking had few independent or interactive effects on the mortality or physiological response of angled-and-released S. ciliata and (2) while hook ingestion caused some mortalities, the protracted physiological effects were limited, with all surviving fish resuming feeding and some eventually ejecting their hooks.

Effects of angling on post-release mortality, gonadal development and somatic condition of Australian bass Macquaria novemaculeata.

This study assessed the protracted effects of two angling treatments (mild and harsh) on the post-release mortality, gonadal development and somatic condition of Australian bass Macquaria novemaculeata in a pond-based experiment. Angled fish were destructively sampled (along with controls) three times: immediately before, and 1 or 4 weeks after release into cages. Compared with the other groups, harshly angled fish had similar, low immediate and short-term mortalities (< or =5%), but significant delayed mortality (25%) and fed only minimally. None of these fish ejected ingested hooks. Forty-six per cent of captive fish (across all groups) and 20% of wild fish had non-ripening gonads (stage I) prior to, or during, the experiment. In females with ripening ovaries (stages II or III), neither angling treatment significantly reduced standardized gonadal mass. The mean per cent of atretic oocytes increased among females in all groups, but was significantly greater in those that were harshly angled. The results demonstrated that the gonadal development of M. novemaculeata could be suppressed or impaired (by angling, handling and confinement), and that further research is warranted. In particular, the timing and severity of angling in relation to the stage of gonadal development could have important implications for the introduction of temporal restrictions to angling.

Development of microsatellites from the rare ironstone endemic, Tetratheca paynterae ssp. paynterae and cross-species amplification.

Nineteen microsatellite markers were developed from Tetratheca paynterae ssp. paynterae, a rare and endangered, leafless, perennial shrub. Twelve loci were polymorphic in T. paynterae ssp. paynterae with two to 14 alleles per locus and mean expected heterozygosity of 0.62. Primer pairs were tested on four other Tetratheca species from ironstone ranges in southern Western Australia. Ten loci were polymorphic in T. paynterae ssp. cremnobata and T. aphylla ssp. aphylla, three in T. harperi and four in T. erubescens. The level of polymorphism was adequate for studies of genetic structure and mating systems in three of the five taxa.

Nuclear RFLP variation in Eucalyptus camaldulensis Dehnh. from northern Australia.

Eucalyptus camaldulensis Dehnh. is the most widely planted eucalypt in the tropics. Natural populations are riparian and sampling strategies for breeding programmes have assumed that gene flow among drainage basins is limited. RFLP variation, within and among 31 populations from river systems across northern Australia, was analysed to test this hypothesis. To allow comparisons within and between river systems, trees were sampled from up to three populations per river system. Allele frequencies were correlated with longitude for more than half the 33 RFLP loci surveyed. Genetic identity was greatest between populations in closest geographic proximity, irrespective of river system, suggesting that sampling strategies for breeding programmes should be based on geographic distance rather than river system. The level of genetic variation was similar throughout the geographic range examined (mean H(E) = 0.49). However, there was evidence of a barrier to gene flow between populations in the east and west of the species range. The RFLP data support morphological evidence of hybridisation between E. camaldulensis and E. tereticornis Sm. in several populations in northeast Queensland and the genetic divergence of E. camaldulensis subsp. simulata Brooker and Kleinig.

Sodium-proton exchange across the apical membrane of the alveolar type II cell of the fetal sheep.

In order to detect and characterise Na(+)-H+ countertransport in the fetal lung epithelium we have studied under a variety of conditions the effect of an outward facing H+ gradient on Na+ uptake into purified apical membrane vesicles prepared from alveolar type II cells. Kinetic analysis of the data reveals both a diffusional and a saturable component of total Na+ uptake. Evidence for the presence of a Na(+)-H+ exchanger is demonstrated by (1) stimulation of Na+ uptake by proton loading of vesicles both in the presence and absence of chemical voltage clamping; (2) saturation kinetics with respect to external Na+ with a Km of 16 mM and a Vmax of 2.1 nmol/mg protein per min; (3) amiloride inhibition of Na+ uptake driven by pH gradient. We conclude that although diffusion may be the major component of total Na+ uptake at physiological external Na+ concentration, Na(+)-H+ countertransport provides a possible mechanism for the acidification of fetal lung liquid in-vivo in addition to its established role in intracellular pH and volume regulation.

Transport of sodium into apical membrane vesicles prepared from fetal sheep alveolar type II cells.

A method is described for isolating apical plasma membrane vesicles from fetal alveolar type II cells. The procedure yields purified apical membranes which are enriched 24-fold with the brush-border enzyme marker, alkaline phosphatase. Contamination of this fraction by basolateral membranes and organelles is minimal. Evidence for transport of Na+ into an intravesicular space is demonstrated by: (1) time-dependent uptake of Na+ with release of accumulated Na+ by treatment with detergent; (2) a linear inverse correlation between Na+ uptake and medium osmolarity. In addition, Na+ uptake is shown to be anion dependent (SCN- greater than Cl- greater than gluconate-) and sensitive to amiloride inhibition at a concentration of 1 mM.